stable donor cell lines expressing ms2 sgrna Search Results


ms 2 bacteriophage  (ATCC)
97
ATCC ms 2 bacteriophage
Ms 2 Bacteriophage, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stable donor cell lines expressing ms2 sgrna  (InvivoGen)
97
InvivoGen stable donor cell lines expressing ms2 sgrna
Stable Donor Cell Lines Expressing Ms2 Sgrna, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ms2 gfp  (Addgene inc)
93
Addgene inc ms2 gfp
Ms2 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stable cell infection lenti ms2 p65 hsf1  (Addgene inc)
93
Addgene inc stable cell infection lenti ms2 p65 hsf1
Stable Cell Infection Lenti Ms2 P65 Hsf1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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u2os 2-6-3 yfp-ms2 stable cells  (American Bioanalytical Inc)
90
American Bioanalytical Inc u2os 2-6-3 yfp-ms2 stable cells
U2os 2 6 3 Yfp Ms2 Stable Cells, supplied by American Bioanalytical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyclin d1  (Proteintech)
96
Proteintech cyclin d1
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Cyclin D1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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dcas9 vp64 ms2 p65 hsf k562 sam cell line  (Addgene inc)
88
Addgene inc dcas9 vp64 ms2 p65 hsf k562 sam cell line
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Dcas9 Vp64 Ms2 P65 Hsf K562 Sam Cell Line, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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activation domain construct ms2 p65 hsf1  (Addgene inc)
96
Addgene inc activation domain construct ms2 p65 hsf1
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Activation Domain Construct Ms2 P65 Hsf1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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dcas9 vp64 blast  (Addgene inc)
95
Addgene inc dcas9 vp64 blast
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Dcas9 Vp64 Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lenti-ms2-p65-hsf1  (Addgene inc)
90
Addgene inc lenti-ms2-p65-hsf1
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Lenti Ms2 P65 Hsf1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lenti-ms2-p65-hsf1/product/Addgene inc
Average 90 stars, based on 1 article reviews
lenti-ms2-p65-hsf1 - by Bioz Stars, 2026-03
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ms2 scans  (Avanti Polar)
94
Avanti Polar ms2 scans
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Ms2 Scans, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ms2 scans - by Bioz Stars, 2026-03
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ms2/ms2 fragmented ions  (Bruker Corporation)
90
Bruker Corporation ms2/ms2 fragmented ions
CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, <t>cyclin-E1/−D1,</t> and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001
Ms2/Ms2 Fragmented Ions, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, cyclin-E1/−D1, and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex

doi: 10.1186/s13046-021-01849-2

Figure Lengend Snippet: CircZFR induced Rb phosphorylation and E2F1 acetylation. a-b. p-Rb S608 and S807, ac-E2F1 K117 and K125, E2F1, cyclin-E1/−D1, and CDK4/2 protein levels were detected in HeLa and SiHa cells overexpressing circZFR, or circ-ZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl and sh-Ctrl, respectively). The relative intensities of these proteins were quantified by ImageJ (right panel). c. p-Rb S608 and S807, ac-E2F1 K117 and K125, cyclin-E1, and CDK2 were detected in tumors (T) and the paired normal tissues (N). d. The relative intensities of each protein in the paired dot plots of 30 cervical cancer patients were detected using ImageJ software. The analyses used a two-tailed paired Student’s t-test. e. The correlation between the expression of circZFR and the cell cycle proteins p-Rb and ac-E2F1. The Pearson correlation coefficients (r) and p values were performed using GraphPad Prism 7.0 software. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

Article Snippet: After blocking, the membranes were immunoblotted with primary antibodies against p-Rb S807 (ab184796, 1:1000, Abcam, Cambridge, UK), p-Rb S608 (ab172975, 1:20000), p-Rb S780 (ab173289, 1:10000), p-Rb T821 (ab32015, 1:5000), p-Rb (ab181616, 1:2000), E2F1 (ab179445, 1:2000), cyclin E1 (ab33911, 1:2000), CDK4 (ab108357, 1:5000), CDK2 (ab32147, 1:5000), ac-E2F1 K117 (YK0087, 1:2000, Immunoway, Texas, USA), ac-E2F1 K125 (YK0088, 1:1000), cyclin D1 (60186–1-Ig, 1:10000, Proteintech, Wuhan, China), SSBP1 (12212–1-AP, 1:1000, Proteintech), and β–actin (60008–1-Ig, 1:20000, Proteintech) overnight at 4 °C.

Techniques: Phospho-proteomics, Knockdown, Software, Two Tailed Test, Expressing

CircZFR promotes CDK2/cyclin-E1 and CDK2/SSBP1 assembly and interacts with SSBP1. a . Model of i n vivo circRNA pull-down using MS2-tagging system, and the following label-free mass spectrometric (MS) analysis. b . Confirmation of the overexpression of circZFR using qRT-PCR analysis. c . Co-transfection of circZFR-MS2 GFP and MS2-CP-Flag mCherry plasmids to induce the expression of MS2 RNA hairpins with overexpressed circZFR and a fusion protein MS2-CP-Flag, which could recognize MS2 RNA hairpins (Scale bar = 200 μm). The green fluorescence-labeled circZFR (up) and the red fluorescence-labeled MS2-CP-Flag (bottom). d . Western Blot test the MS2-CP-Flag pulled down by anti-Flag (up). The enrichment of circZFR in the complex with MS2-CP-Flag was detected by qRT-PCR (bottom). The assays were done twice in triplicate. e . The protein complex with MS2-CP-Flag was tested using a label-free MS. The peptides were matched to SSBP1. f . circRNA immunoprecipitation (circRIP) assay to measure the amount of circZFR pulled down by SSBP1 and IgG antibodies in the HeLa and SiHa cells stably overexpressed circZFR and circ-Ctrl. The assays were done twice in triplicate. g . The lysates prepared from the HeLa cells stably overexpressing circZFR, or circZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl, and sh-Ctrl, respectively) were subjected to immunoprecipitation using anti-CDK2. ** P < 0.001, **** P < 0.0001, MS2-CP: MS2 bacteriophage coat protein, MS: mass spectrometric, GFP: green fluorescent protein, circRIP: circRNA immunoprecipitation

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The circular RNA circZFR phosphorylates Rb promoting cervical cancer progression by regulating the SSBP1/CDK2/cyclin E1 complex

doi: 10.1186/s13046-021-01849-2

Figure Lengend Snippet: CircZFR promotes CDK2/cyclin-E1 and CDK2/SSBP1 assembly and interacts with SSBP1. a . Model of i n vivo circRNA pull-down using MS2-tagging system, and the following label-free mass spectrometric (MS) analysis. b . Confirmation of the overexpression of circZFR using qRT-PCR analysis. c . Co-transfection of circZFR-MS2 GFP and MS2-CP-Flag mCherry plasmids to induce the expression of MS2 RNA hairpins with overexpressed circZFR and a fusion protein MS2-CP-Flag, which could recognize MS2 RNA hairpins (Scale bar = 200 μm). The green fluorescence-labeled circZFR (up) and the red fluorescence-labeled MS2-CP-Flag (bottom). d . Western Blot test the MS2-CP-Flag pulled down by anti-Flag (up). The enrichment of circZFR in the complex with MS2-CP-Flag was detected by qRT-PCR (bottom). The assays were done twice in triplicate. e . The protein complex with MS2-CP-Flag was tested using a label-free MS. The peptides were matched to SSBP1. f . circRNA immunoprecipitation (circRIP) assay to measure the amount of circZFR pulled down by SSBP1 and IgG antibodies in the HeLa and SiHa cells stably overexpressed circZFR and circ-Ctrl. The assays were done twice in triplicate. g . The lysates prepared from the HeLa cells stably overexpressing circZFR, or circZFR knockdown (sh-circ) and the corresponding controls (circ-Ctrl, and sh-Ctrl, respectively) were subjected to immunoprecipitation using anti-CDK2. ** P < 0.001, **** P < 0.0001, MS2-CP: MS2 bacteriophage coat protein, MS: mass spectrometric, GFP: green fluorescent protein, circRIP: circRNA immunoprecipitation

Article Snippet: After blocking, the membranes were immunoblotted with primary antibodies against p-Rb S807 (ab184796, 1:1000, Abcam, Cambridge, UK), p-Rb S608 (ab172975, 1:20000), p-Rb S780 (ab173289, 1:10000), p-Rb T821 (ab32015, 1:5000), p-Rb (ab181616, 1:2000), E2F1 (ab179445, 1:2000), cyclin E1 (ab33911, 1:2000), CDK4 (ab108357, 1:5000), CDK2 (ab32147, 1:5000), ac-E2F1 K117 (YK0087, 1:2000, Immunoway, Texas, USA), ac-E2F1 K125 (YK0088, 1:1000), cyclin D1 (60186–1-Ig, 1:10000, Proteintech, Wuhan, China), SSBP1 (12212–1-AP, 1:1000, Proteintech), and β–actin (60008–1-Ig, 1:20000, Proteintech) overnight at 4 °C.

Techniques: MS2 Tagging, Over Expression, Quantitative RT-PCR, Cotransfection, Expressing, Fluorescence, Labeling, Western Blot, Immunoprecipitation, Stable Transfection, Knockdown